PFKM Protein, Human, Recombinant (His & GST Tag)

Product Specifications

Background

PFK1, also known as PFKM, is a regulatory glycolytic enzyme. PFK1 converts fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. It is a muscle-type isozyme. There are three phosphofructokinase isozymes in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Mutations in PFK1 gene have been related to glycogen storage disease type VII, also identified as Tarui disease.

Overview

A DNA sequence encoding the human PFKM (P08237-1) (Thr2-Val780) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Synonyms

ATP-PFK Protein, Human; GSD7 Protein, Human; PFK-1 Protein, Human; PFK1 Protein, Human; PFKA Protein, Human; PFKX Protein, Human; PPP1R122 Protein, Human

Gene Name

Phosphofructokinase, muscle

UniProt

P08237-1

Expression System

Baculovirus-Insect Cells

Tag

N-GST & His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Kinase activity untested

Form

Liquid

Buffer

Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins

Molecular Weight

The recombinant human PFKM /GST chimera consists of 1016 amino acids and has a calculated molecular mass of 112.9 kDa. The recombinant protein migrates as an approximately 113 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.