Cardiotrophin 1 Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

CTF1 is the first HOX11 protein partner identified that plays an important role in hematopoietic precursor cell immortalization. CTF1 was found to protect a gene from silencing when its DNA-binding sites were interposed between the gene and the telomeric extremity, while it did not affect a gene adjacent to the telomere. Protein fusions containing the CTF1 histone-binding domain displayed similar activities, while mutants impaired in their ability to interact with the histone did not. Cardiotrophin-1 (CTF1) has been reported to act as a trophic factor for a few neurons, such as sensory, cholinergic, dopaminergic, motor and cortical neurons. Studies have indicated that CTF1 delays degenerative disease progression in motor neuron disease.

Overview

A DNA sequence encoding the human CTF1 (NP_001321.1) (Ser2-Ala201) was expressed with the fused Fc region of human IgG1 at the N-terminus.

Synonyms

CT-1 Protein, Human; CT1 Protein, Human

Gene Name

Cardiotrophin 1

Expression System

HEK293 Cells

Tag

N-hFc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The EDfor this effect is typically 0.015-0.06 μg/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Interleukin proteins, Biological Target proteins

Molecular Weight

The recombinant human CTF1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 460 amino acids and has a predicted molecular mass of 49.5 kDa. The apparent molecular mass of the protein is approximately 54 and 37 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.