Chorionic Gonadotropin/CGA Protein, Human, Recombinant (mFc Tag)

Product Specifications

Overview

A DNA sequence encoding the human CGA (NP_000726.1) (Met1-Ser116) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Synonyms

CG-ALPHA Protein, Human; FSH alpha Protein, Human; FSHA Protein, Human; GPHa Protein, Human; GPHA1 Protein, Human; HCG Protein, Human; LHA Protein, Human; TSHA Protein, Human

Gene Name

Glycoprotein hormones, alpha polypeptide

Expression System

HEK293 Cells

Tag

C-mFc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Diagnostic proteins, Biomarker Proteins

Molecular Weight

The recombinant human CGA/mFc comprises 326 amino acids and has a predicted molecular mass of 36.6 kDa. The apparent molecular mass of the protein is approximately 45.8 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.