MUC1 Protein, Human, Recombinant (mFc Tag)

Mucin 1, cell surface-associated (MUC1) or polymorphic epithelial mucin (PEM) is a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach, and pancreas. MUC-1/CC1/CD227 Exclusively located in the apical domain of the plasma membrane of highly polarized epithelial cells. After endocytosis, internalized, and recycled to the cell membrane. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. The alpha subunit has cell adhesive properties. MUC-1/CC1/CD227 Can act both as an adhesion and an anti-adhesion protein. This protein May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. MUC-1/CC1/CD227 participated in modulates signaling in ERK, SRC, and NF-kappa-B pathways. In the activated T-cells, MUC-1/CC1/CD227 influences directly or indirectly the Ras/MAPK pathway. MUC-1/CC1/CD227 Promotes tumor progression and regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

ADMCKD Protein, Human; ADMCKD1 Protein, Human; CA15-3 Protein, Human; CD227 Protein, Human; EMA Protein, Human; H23AG Protein, Human; KL-6 Protein, Human; MAM6 Protein, Human; MCD Protein, Human; MCKD Protein, Human; MCKD1 Protein, Human; MUC-1 Protein, Human; MUC-1/SEC Protein, Human; MUC-1/X Protein, Human; MUC1/ZD Protein, Human; Mucin 1 Protein, Human; PEM Protein, Human; PEMT Protein, Human; PUM Protein, Human

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

CD Molecule proteins, CAR T Cell Therapy Target Proteins, Cancer proteins, Immunotherapy proteins, Biomarker Proteins, Biological Target proteins, Diagnostic proteins

The recombinant human MUC1/mFc is a disulfide-linked homodimer. The reduced monomer consists of 369 amino acids after removal the signal peptide and has a predicted molecular mass of 41 kDa. As a result of glycosylation, rhMUC1/mFc monomer migrates as an approximately 50.2 and 34.8 kDa band in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the human MUC1 isoform 2 (NP_001018016.1) extracellular domain (Met1-Gly167) was fused with the Fc region of mouse IgG at the C-terminus.