FGF4 Protein, Human, Recombinant

Product Specifications

Background

FGF (fibroblast growth factor) signalling is known to be required for many aspects of mesoderm formation and patterning during Xenopus development and has been implicated in regulating genes required for the specification of both blood and skeletal muscle lineages. Fibroblast growth factor 4 (FGF4) signaling induces differentiation from embryonic stem cells (ESCs) via the phosphorylation of downstream molecules such as mitogen-activated protein kinase/extracellular signal-related kinase (MEK) and extracellular signal-related kinase 1/2 (ERK1/2) . Fibroblast Growth Factor 4 (FGF-4) could not only increase the proliferation of bone marrow mesenchymal stem cells (BMSCs), but also induce BMSCs into hepatocyte-like cells in vitro. FGF4 transduced BMSCs contributed to liver regeneration might by the transplanted microenvironment. The FGF4-bFGF BMSCs thus can enhance the survival of the transplanted cells, diminish myocardial fibrosis, promote myocardial angiogenesis, and improve cardiac functions.

Overview

A DNA sequence encoding the human FGF4 (NP_001998.1) (Ser54-Leu206) was expressed.

Synonyms

HBGF-4 Protein, Human; HST Protein, Human; HST-1 Protein, Human; HSTF1 Protein, Human; K-FGF Protein, Human; KFGF Protein, Human

Gene Name

Fibroblast growth factor 4

Expression System

E. coli

Tag

No tag

Endotoxin

Please contact us for more information.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The EDfor this effect is typically 0.04-0.2 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cell Therapy proteins

Molecular Weight

The recombinant human FGF4 consists of 153 amino acids and predicts a molecular mass of 16.8 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.