CD40 Protein, Human, Recombinant (ECD, hFc Tag)

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3) . In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

1. Measured by its binding ability in a functional ELISA. 2. Immobilized human CD40L-His (Cat:PH52274E2) at 10 μg/mL (100 μL/well) can bind Human CD40-Fc. The ECof Human CD40-Fc is 10-30 ng/mL.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Cytokine Proteins, epigenetic proteins, Immune Checkpoint Proteins, Biological Target proteins, TNF Superfamily proteins, CD Molecule proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins

The recombinant human CD40 consists 414 amino acids and predicts a molecular mass of 46.2 kDa. As a result of glycosylation, the apparent molecular mass of human CD40 is approximately 54.1 kDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.