ZIP Kinase/DAPK3 Protein, Human, Recombinant (GST Tag)

Product Specifications

Background

Death-associated protein kinase 3, also known as DAP kinase 3, ZIP-kinase, DAPK3 and ZIPK, is a nucleus and cytoplasm protein which belongs to theprotein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK3 / ZIPK contains oneprotein kinase domain. It is a serine/threonine kinase which acts as a positive regulator of apoptosis. It phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. DAPK3 / ZIPK is a homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. It also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. DAPK3 / ZIPK regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It is involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.

Overview

A DNA sequence encoding the full length of human DAPK3 (NP_001339.1) (Met 1-Arg 454) was fused with the GST tag at the N-terminus.

Synonyms

DLK Protein, Human; ZIP Protein, Human; ZIPK Protein, Human

Gene Name

Death-associated protein kinase 3

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by SDS-PAGE.

Activity

The specific activity was determined to be 5 nmol/min/mg using MBP as substrate.

Form

Liquid

Buffer

Supplied as sterile 20mM Tris, 500mM NaCl, 10mM GSH, pH 7.4 Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Function

Kinase proteins, Enzyme Proteins

Molecular Weight

The recombinant human DAPK3/GST chimera consists of 678 amino acids and predicts a molecular mass of 79 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.