IL17 Protein, Human, Recombinant

Product Specifications

Background

IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO) . High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.

Overview

A DNA sequence encoding the human IL17A (Q16552) (Ile20-Ala155) was expressed, with a N-terminal Met.

Synonyms

CTLA-8 Protein, Human; CTLA8 Protein, Human; IL-17 Protein, Human; IL-17A Protein, Human; IL17 Protein, Human

Gene Name

Interleukin 17A

UniProt

Q16552

Expression System

E. coli

Tag

No tag

Field of Research

Neuroinflammation

Endotoxin

Please contact us for more information.

Purity

> 97 % as determined by SDS-PAGE.

Activity

Measured by its ability to induce IL-6 secretion by NIH 3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα. The EDfor this effect is 0.5-2.5 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Biological Target proteins, Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Immunotherapy proteins, Cell Therapy proteins

Molecular Weight

The recombinant human IL17A consists of 137 amino acids and predicts a molecular mass of 15.7 KDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.