CHRNA5 Protein, Human, Recombinant (His Tag)
Product Specifications
Background
Genetic variation in the cluster on chromosome 15, encoding the nicotinic acetylcholine receptor subunits (CHRNA5-CHRNA3-CHRNB4), has shown strong associations with tobacco consumption and an additional risk increase in smoking-related diseases such as chronic obstructive pulmonary disease (COPD), peripheral artery disease and lung cancer. CHRNA5 may influence susceptibility to lung cancer among Han smokers. Genetic variation in nicotinic receptor alpha 5 (CHRNA5) has been associated with increased risk of addiction-associated phenotypes in humans.
Synonyms
LNCR2 Protein, Human
Gene Name
Cholinergic receptor, nicotinic, alpha 5 (neuronal)
Expression System
HEK293 Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant human CHRNA5 consists 243 amino acids and predicts a molecular mass of 28 kDa.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH51350M2.pdf
Symbol
CHRNA5
Species
Human
Protein ID
NP_000736.2
Overview
A DNA sequence encoding the human CHRNA5 (NP_000736.2) (Met1-Thr254) was expressed with a polyhistidine tag at the C-terminus.
AA Sequence
Met1-Thr254
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_000736.2
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