PD-L1 Protein, Human, Recombinant (ECD, His Tag), Biotinylated

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

B7-H Protein, Human; B7-H1 Protein, Human; B7H1 Protein, Human; PD-L1 Protein, Human; PDCD1L1 Protein, Human; PDCD1LG1 Protein, Human; PDL1 Protein, Human

Measured by its binding ability in a functional ELISA. Immobilized Human PD-1 His & hFc (Cat:10377-H03H) at 2 μg/ml (100 μl/well) can bind Human PD-L1 His, Biotinylated (Cat:PH51165M2-B), the ECof Human PD-L1 His, Biotinylated is 50-300 ng/mL.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Immune Checkpoint Proteins, Biological Target proteins, Biotinylated proteins, B7 Family proteins, CD Molecule proteins, CAR T Cell Therapy Target Proteins, Cancer proteins, Immunotherapy proteins

The recombinant mature human B7-H1 comprises 232 amino acids and predicts a molecular mass of 26.8 kDa. As a result of glycosylation, the human B7-H1 migrates as an approximately 35-38 kDa protein in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the N-terminal segment (Met1-Thr239) of the extracellular domain of human B7-H1 (NP_054862.1) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.