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GLT8D2 Protein, Human, Recombinant (His Tag)

Product Specifications

Background

GLT8D2 is a glycosyltransferase of apoB100 that regulates apoB100 levels in hepatocytes. GLT8D2 expression increased in steatosis HepG2 cells compared with that in normal HepG2 cells. GLT8D2 participated in nonalcoholic fatty liver disease (NAFLD) pathogenesis possibly by negatively regulating MTP expression.

Overview

A DNA sequence encoding the human GLT8D2 (NP_112592.1) (Lys25-Ser349) was expressed with a polyhistidine tag at the N-terminus.

Synonyms

UNQ1901/PRO4347 Protein, Human

Gene Name

Glycosyltransferase 8 domain containing 2

Expression System

Baculovirus-Insect Cells

Tag

N-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant human GLT8D2 consists 344 amino acids and predicts a molecular mass of 39.6 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Symbol

GLT8D2

Species

Human

Protein ID

NP_112592.1

AA Sequence

Lys25-Ser349

Protein ID Link

https://www.ncbi.nlm.nih.gov/protein/NP_112592.1

Frequently Asked Questions

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