Prolactin Protein, Human, Recombinant (His Tag)

Product Specifications

Background

Prolactin (PRL) is a hormone with multiple actions in the central nervous system (CNS) spanning from physiology to pathology. PRL exerts different actions through its receptors that can be found in both neurons and glial cells (astrocytes, microglia and oligodendrocytes) of the brain. It is generally believed that in vertebrates, prolactin (PRL) is predominantly synthesized and released by pituitary lactotrophs and plays important roles in many physiological processes via activation of PRL receptor (PRLR), including water and electrolyte balance, reproduction, growth and development, metabolism, immuno-modulation, and behavior.

Overview

A DNA sequence encoding the human PRL (CAA25214.1) (Met1-Cys227) was expressed with a polyhistidine tag at the C-terminus.

Gene Name

Prolactin

Expression System

Baculovirus-Insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured by its ability to promote proliferation of INS-1 cells. The EDfor this effect is typically 20-120 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 %glycerol, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Biological Target proteins, Diagnostic proteins, Biomarker Proteins

Molecular Weight

The recombinant human PRL consists of 210 amino acids and predicts a molecular mass of 24.3 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.