Prolactin Protein, Human, Recombinant (His Tag)
Product Specifications
Background
Prolactin (PRL) is a hormone with multiple actions in the central nervous system (CNS) spanning from physiology to pathology. PRL exerts different actions through its receptors that can be found in both neurons and glial cells (astrocytes, microglia and oligodendrocytes) of the brain. It is generally believed that in vertebrates, prolactin (PRL) is predominantly synthesized and released by pituitary lactotrophs and plays important roles in many physiological processes via activation of PRL receptor (PRLR), including water and electrolyte balance, reproduction, growth and development, metabolism, immuno-modulation, and behavior.
Overview
A DNA sequence encoding the human PRL (CAA25214.1) (Met1-Cys227) was expressed with a polyhistidine tag at the C-terminus.
Gene Name
Prolactin
Expression System
Baculovirus-Insect Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 90 % as determined by SDS-PAGE.
Activity
Measured by its ability to promote proliferation of INS-1 cells. The EDfor this effect is typically 20-120 ng/mL.
Form
Lyophilized
Buffer
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 %glycerol, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Cytokine Proteins, Biological Target proteins, Diagnostic proteins, Biomarker Proteins
Molecular Weight
The recombinant human PRL consists of 210 amino acids and predicts a molecular mass of 24.3 kDa.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
PRL
Species
Human
Protein ID
CAA25214.1
AA Sequence
Met1-Cys227
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/CAA25214.1
Frequently Asked Questions
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