PD-1 Protein, Human, Recombinant (ECD, His Tag)

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

1. Immobilized human PD-1 (Cat:PH51080M2) at 2 μg/ml (100 μl/well) can bind PD-L1 Protein, Human, Recombinant (ECD, hFc Tag) (Cat:PH51110M4), the ECof human B7-H1 is 150-600 ng/mL. 2. Immobilized human PD-1 (Cat:PH51080M2) at 2 μg/mL (100 μL/well) can bind Anti-PD1 (MDX) -IgG4 Antibody (Nivolumab) (Cat:68050-H001), the ECis 8-60 ng/mL. 3. Labeled biotin to PD-1 Protein, Human, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of PD-1 Protein, Human, Recombinant (His Tag), Biotinylated (Cat. PH50474M2) bound to Keytruda was 0.1 nM.

Lyophilized from sterile 50mM MOPS, 500mM NaCl, pH 7.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

B7 Family proteins, CD Molecule proteins, CAR T Cell Therapy Target Proteins, Cancer proteins, Receptor Proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins, Diagnostic proteins

The recombinant mature human PD1 consists of 154 amino acids and has a calculated molecular mass of 17.4 kDa. As a result of glycosylation, human PD1 migrates as an approximately 41.1 kDa protein in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.