Peroxiredoxin 6 Protein, Human, Recombinant (His Tag)
Product Specifications
Background
PRDX6, a member of antioxidant protein superfamily, plays an important role in oxidative stress, catabolism of lipids and phospholipid lipisomes. Peroxiredoxin 6 (PRDX6) is involved in redox regulation of the cell and is thought to be protective against oxidant injury. Peroxiredoxin 6 (PRDX6) is a bifunctional protein with both glutathione peroxidase (GPx) and iPLA2 activities, which are concomitantly increased with the expression of PRDX6. PRDX6 promoted lung tumor growth in an in vivo allograft model.
Overview
A DNA sequence encoding the human PRDX6 (P30041) (Met 1-Pro 224) was expressed, with a polyhistidine tag at the N-terminus.
Synonyms
1-Cys Protein, Human; aiPLA2 Protein, Human; AOP2 Protein, Human; HEL-S-128m Protein, Human; NSGPx Protein, Human; p29 Protein, Human; PRX Protein, Human
Gene Name
Peroxiredoxin 6
UniProt
P30041
Expression System
E. coli
Tag
N-His
Endotoxin
Please contact us for more information.
Purity
> 95 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Enzyme Proteins
Molecular Weight
The recombinant human PRDX6 comprises 235 amino acids and has a predicted molecular mass of 26.5 kDa as estimated in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
PRDX6
Species
Human
Uniprot URL
https://www.uniprot.org/uniprot/P30041
AA Sequence
Met1-Pro224
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