FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag), Biotinylated

Product Specifications

Overview

A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (P55899) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified. The purified protein was biotinylated in vitro.

Synonyms

Alpha-chain Protein, Human; FCRN Protein, Human

Gene Name

Fc fragment of IgG, receptor, transporter, alpha

UniProt

P55899&P61769

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Fc Receptors

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (His Tag), Biotinylated (Cat. PH51041M2) bound to IgG1 Antibody was 4.6 nM.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Fc receptor proteins, Biotinylated proteins, Receptor Proteins

Molecular Weight

The recombinant heterodimer of human FCGRT/B2M consists of 385 (285 + 100) amino acids and predicts a molecular mass of 43.5 (31.8 + 11.7) kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.