LIGHT Protein, Human, Recombinant (rFc Tag)

Product Specifications

Background

LIGHT, also known as TNFSF14 or CD258, is a newly identified member of the TNF superfamily (TNFSF14) that is expressed by activated T lymphocytes, monocytes, granulocytes, spleen cells, and immature dendritic cells. TNFSF14 / LIGHT / CD258 is a type II transmembrane protein that is known to bind 2 membrane-bound TNFSF signaling receptors: HVEM, which is predominantly expressed by T cells, and lymphotoxin β receptor (LTβR), which is expressed by stromal cells and nonlymphoid hematopoietic cells. TNFSF14 / LIGHT / CD258 also binds to a soluble non-signaling receptor, decoy receptor 3 (DcR3), which can modulate the function of LIGHT in vivo. TNFSF14 / LIGHT / CD258 can also costimulate T cell responses via HVEM, which is constitutively expressed in most lymphocyte subpopulations, including CD4and CDT cells. In addition, TNFSF14 / LIGHT / CD258 has been shown to suppress tumor formation in vivo and to induce tumor cell apoptosis via the up-regulation of intercellular adhesion molecule 1 and an increased lymphocyte adhesion to cancer cells. Thus, TNFSF14 / LIGHT / CD258 is being actively investigated as a possible basis for cancer treatment.

Overview

A DNA sequence encoding the human TNFSF14 (AAK26160.1) (Asp74-Val240) was expressed with the Fc region of rabbit IgG at the N-terminus..

Synonyms

CD258 Protein, Human; HVEML Protein, Human; LIGHT Protein, Human; LTg Protein, Human; TR2 Protein, Human

Gene Name

Tumor necrosis factor (ligand) superfamily, member 14

Expression System

HEK293 Cells

Tag

N-rFc

Field of Research

Cancer Drug Targets

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) . The EDfor this effect is typically 5-25 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

TNF Superfamily proteins, CD Molecule proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins, Cytokine Proteins, Growth Factor proteins

Molecular Weight

The recombinant human TNFSF14 consists of 418 amino acids and predicts a molecular mass of 45.7 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.