IL-23 Protein, Human, Recombinant (His & AVI Tag), Biotinylated

Product Specifications

Overview

A DNA sequence encoding the human IL23 complex composed of human IL12B (NP_002178.2) (Met1-Ser328) and human IL23A (NP_057668.1) (Arg20-Pro189) linked by a polypeptide linker was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Synonyms

IL-23 Protein, Human; IL-23A Protein, Human; IL23 Protein, Human; IL23P19 Protein, Human; P19 Protein, Human; SGRF Protein, Human

Expression System

HEK293 Cells

Tag

C-AVI & His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Immobilized Human IL23 Receptor Protein (ECD, hFc Tag) (Cat:PH51521M4) at 2 μg/mL (100 μL/well) can bind Human IL-23 Protein (His & AVI Tag), Biotinylated (Cat:PH51020M9), the ECis 80-480ng/mL.

Form

Liquid

Buffer

Supplied as sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Function

Biotinylated proteins

Molecular Weight

The recombinant human IL23 complex (IL23A&IL12B) consists of 517 amino acids and predicts a molecular mass of 57.6 kDa.

Storage Conditions

Liquid. It is shipped out with blue ice. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.