IL-23 Protein, Human, Recombinant (His & AVI Tag), Biotinylated
Product Specifications
Synonyms
IL-23 Protein, Human; IL-23A Protein, Human; IL23 Protein, Human; IL23P19 Protein, Human; P19 Protein, Human; SGRF Protein, Human
Expression System
HEK293 Cells
Tag
C-AVI & His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
Immobilized Human IL23 Receptor Protein (ECD, hFc Tag) (Cat:PH51521M4) at 2 μg/mL (100 μL/well) can bind Human IL-23 Protein (His & AVI Tag), Biotinylated (Cat:PH51020M9), the ECis 80-480ng/mL.
Form
Liquid
Buffer
Supplied as sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Function
Biotinylated proteins
Molecular Weight
The recombinant human IL23 complex (IL23A&IL12B) consists of 517 amino acids and predicts a molecular mass of 57.6 kDa.
Storage Conditions
Liquid. It is shipped out with blue ice. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH51020M9.pdf
Symbol
IL12B & IL23A
Species
Human
Protein ID
NP_002178.2&NP_057668.1
Overview
A DNA sequence encoding the human IL23 complex composed of human IL12B (NP_002178.2) (Met1-Ser328) and human IL23A (NP_057668.1) (Arg20-Pro189) linked by a polypeptide linker was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
AA Sequence
Met1-Ser328 & Arg20-Pro189
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_002178.2&NP_057668.1
Available Sizes
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