DDR1 Protein, Human, Recombinant (aa 444-913, His & GST Tag)

Product Specifications

Background

Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.

Overview

A DNA sequence encoding the human DDR1 (Q08345-1) (Arg444-Val913) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Synonyms

CAK Protein, Human; CD167 Protein, Human; DDR Protein, Human; EDDR1 Protein, Human; HGK2 Protein, Human; MCK10 Protein, Human; NEP Protein, Human; NTRK4 Protein, Human; PTK3 Protein, Human; PTK3A Protein, Human; RTK6 Protein, Human; TRKE Protein, Human

Gene Name

Discoidin domain receptor tyrosine kinase 1

UniProt

Q08345-1

Expression System

Baculovirus-Insect Cells

Tag

N-GST & His

Field of Research

Receptor Tyrosine Kinases (RTKs)

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by SDS-PAGE.

Activity

The specific activity was determined to be 2.75 nmol/min/mg using synthetic AXLtide peptide (CKKSRGDYMTMQIG) as substrate.

Form

Liquid

Buffer

Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Function

Kinase proteins, CD Molecule proteins, Enzyme Proteins

Molecular Weight

The recombinant human DDR1 /GST chimera consists of 707 amino acids and has a calculated molecular mass of 80 kDa. The recombinant protein migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.