VEGF121b Protein, Human, Recombinant

Product Specifications

Background

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF) /vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.

Overview

A DNA sequence encoding the human VEGF 121 isoform b (P15692-9) (Met1-Asp147, with last 6 amino acids switch from CDKPRRtoSLTRKD) was expressed.

Synonyms

MVCD1 Protein, Human; VEGF Protein, Human; VEGF165 Protein, Human; VPF Protein, Human

Gene Name

Vascular endothelial growth factor A

UniProt

P15692-9

Expression System

HEK293 Cells

Tag

No tag

Field of Research

Cancer Drug Targets

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

1. Measured by its binding ability in a functional ELISA. Immobilized human VEGF121b (Cat:PH50969M0) at 10μg/mL (100μL/well) can bind human VEGFR2-Fc (Cat:PH50475M4), the ECof human VEGFR2-Fc is 0.06-0.1 μg/mL. 2. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) . The EDfor this effect is typically 1-5ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Growth Factor proteins, Cancer proteins, Immunotherapy proteins, Biological Target proteins, Diagnostic proteins, Biomarker Proteins, Cell Therapy proteins

Molecular Weight

The recombinant human VEGF consists of 121 amino acids and has a predicted molecular mass of 14 kDa. The apparent molecular mass of the human VEGF121b is approximately 20 and 16 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.