DKK1 Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Overview

A DNA sequence encoding the human DKK1 (NP_036374.1) (Met2-His266) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

DKK-1 Protein, Human; SK Protein, Human

Gene Name

Dickkopf WNT signaling pathway inhibitor 1

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells.The EDfor this effect is approximately 0.01-0.07μg/mL in the presence of 10 ng/mL of mouse Wnt3a.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Growth Factor proteins, Biological Target proteins, Cell Therapy proteins, Cytokine Proteins

Molecular Weight

The recombinant human DKK1 consists of 473 amino acids and predicts a molecular mass of 52.5 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.