HER3/ERBB3 Protein, Human, Recombinant (ECD, His Tag)

ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.

C-erbB-3 Protein, Human; c-erbB3 Protein, Human; EEBB3 Protein, Human; ErbB-3 Protein, Human; erbB3-S Protein, Human; HER3 Protein, Human; LCCS2 Protein, Human; MDA-BF-1 Protein, Human; p180-ErbB3 Protein, Human; p45-sErbB3 Protein, Human; p85-sErbB3 Protein, Human

1. Measured by its binding ability in a functional ELISA. 2. Immobilized human ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The ECof human NRG1 (isoform Beta1) is 0.43 μg/mL.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Kinase proteins, Diagnostic proteins, Growth Factor proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins, Cytokine Proteins, Enzyme Proteins, Biological Target proteins, Biomarker Proteins

The recombinant human ErbB3 protein consists of 635 amino acids after the removal of signal peptide and has a calculated molecular mass of 70.2 kDa. As a result of glycosylation, the recombinant ErbB3 migrates as an approximately 100-110 kDa band in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the extracellular domain (Met 1-Thr 643) of human ErbB3 (NP_001973.2) precursor was expressed, with a C-terminal polyhistidine tag.