Procalcitonin/CALCA Protein, Human, Recombinant (mFc Tag)
Product Specifications
Overview
A DNA sequence encoding the human CALCA (NP_001732.1) (Met1-Asn141) was expressed with the Fc region of mouse IgG1 at the C-terminus.
Synonyms
CALC1 Protein, Human; CGRP Protein, Human; CGRP-I Protein, Human; CGRP1 Protein, Human; CT Protein, Human; KC Protein, Human; PCT Protein, Human; Procalcitonin Protein, Human
Gene Name
Calcitonin-related polypeptide alpha
Expression System
HEK293 Cells
Tag
C-mFc
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Diagnostic proteins
Molecular Weight
The recombinant human CALCA/mFc comprises 350 amino acids and has a predicted molecular mass of 39.2 kDa. The apparent molecular mass of the monomer is approximately 41.6 and 31.2 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
CALCA
Species
Human
Protein ID
NP_001732.1
AA Sequence
Met1-Asn141
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_001732.1
Frequently Asked Questions
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