CD45 Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexes or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.

Overview

A DNA sequence encoding the human CD45 (NP_563578.2) (Met1-Lys416) was fused with the Fc region of human IgG1 at the C-terminus.

Synonyms

B220 Protein, Human; CD45 Protein, Human; CD45R Protein, Human; GP180 Protein, Human; L-CA Protein, Human; LCA Protein, Human; LY5 Protein, Human; T200 Protein, Human

Gene Name

Protein tyrosine phosphatase, receptor type, C

Expression System

HEK293 Cells

Tag

C-hFc

Field of Research

Phosphatases and Regulators

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured by its ability to bind biotinylated human Galectin-1 (Cat:PH51598E0-E) in a functional ELISA.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins, Diagnostic proteins, CD Molecule proteins

Molecular Weight

The recombinant mature human CD45/Fc chimeric protein consists of 629 amino acids and has a calculated molecular mass of 71.1 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human CD45/Fc monomer is approximately 122 kDa due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.