What is the catalog number for GM-CSF Receptor alpha Protein, Human, Recombinant (hFc Tag)?

The catalog number for GM-CSF Receptor alpha Protein, Human, Recombinant (hFc Tag) is PH50859M4.

What sizes are available for GM-CSF Receptor alpha Protein, Human, Recombinant (hFc Tag)?

GM-CSF Receptor alpha Protein, Human, Recombinant (hFc Tag) is available in the following size(s): 100 µg, 1 mg.

How many pack sizes does GM-CSF Receptor alpha Protein, Human, Recombinant (hFc Tag) come in?

GM-CSF Receptor alpha Protein, Human, Recombinant (hFc Tag) is available in 2 different pack sizes.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

GM-CSF Receptor alpha Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

CD116/GM-CSFR has been preferentially associated with M4, M5 subtype of AML but is not specific. The cluster of differentiation (cluster of designation) (often abbreviated as CD) is a protocol used for the identification and investigation of cell surface molecules present on white blood cells initially but found in almost any kind of cell of the body, providing targets for immunophenotyping of cells. Physiologically, CD molecules can act in numerous ways, often acting as receptors or ligands (the molecule that activates a receptor) important to the cell. A signal cascade is usually initiated, altering the behavior of the cell (see cell signaling) . Some CD proteins do not play a role in cell signaling, but have other functions, such as cell adhesion. CD116/GM-CSFR is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. CD116/GM-CSFR is found in the pseudoautosomal region (PAR) of the X and Y chromosomes.

Overview

A DNA sequence encoding the extracellular domain (Met 1-Gly 320) of human GM-CSFRα (NP_006131.2) pro-protein was expressed with the C-terminal fused Fc region of human IgG1.

Synonyms

CD116 Protein, Human; CDw116 Protein, Human; CSF2R Protein, Human; CSF2RAX Protein, Human; CSF2RAY Protein, Human; CSF2RX Protein, Human; CSF2RY Protein, Human; GM-CSF-R-alpha Protein, Human; GMCSFR Protein, Human; GMR Protein, Human; SMDP4 Protein, Human

Gene Name

Colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured by its ability to inhibit GM-CSF dependent proliferation of TF-1 human erythroleukemic cells. The EDfor this effect is typically 10-15 μg/ml.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Interleukin proteins, CD Molecule proteins, Receptor Proteins

Molecular Weight

The recombinant human GM-CSFRα consists of 536 amino acids and has a predicted molecular mass of 61.2 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.