PLA2G2D Protein, Human, Recombinant (hFc Tag)
Product Specifications
Synonyms
PLA2IID Protein, Human; sPLA2-IID Protein, Human; sPLA2S Protein, Human; SPLASH Protein, Human
Gene Name
Phospholipase A2, group IID
UniProt
Q9UNK4
Expression System
HEK293 Cells
Tag
C-hFc
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Enzyme Proteins
Molecular Weight
The recombinant human PLA2G2D/Fc is a disulfide-linked homodimer. The reduced monomer comprises 366 amino acids and has a predicted molecular mass of 41.5 kDa. The apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH50832M4.pdf
Symbol
PLA2G2D
Species
Human
Overview
A DNA sequence encoding the human PLA2G2D (Q9UNK4) (Met1-Cys145) was expressed with the Fc region of human IgG1 at the C-terminus.
Uniprot URL
https://www.uniprot.org/uniprot/Q9UNK4
AA Sequence
Met1-Cys145
Available Sizes
Curated Selection
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