14-3-3 eta/YWHAH Protein, Human, Recombinant (GST Tag)
Product Specifications
Background
YWHAH, the gene encoding the 14-3-3eta isoform, is highly expressed in retinal ganglion cells (RGC) . YWHAH is a positional and functional candidate gene for both schizophrenia and bipolar disorder (BP) . It is located on chromosome 22q12.3, a region that has been implicated by linkage studies in both BP and schizophrenia.
Synonyms
YWHA1 Protein, Human
Gene Name
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
UniProt
Q04917
Expression System
E. coli
Tag
N-GST
Field of Research
Phospho-Serine/phospho-Threonine Binding Proteins Adaptor Proteins Apoptosis Adaptor Proteins
Endotoxin
Please contact us for more information.
Purity
> 90 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile 20mM Tris, 0.15M NaCl, 20mM GST, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant human YWHAH/GST chimera consists of 476 amino acids and has a predicted molecular mass of 55 kDa as estimated in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH50806E6.pdf
Symbol
YWHAH
Species
Human
Overview
A DNA sequence encoding the human YWHAH (Q04917) (Gly 2-Asn 246) was fused with the GST tag at the N-terminus.
Uniprot URL
https://www.uniprot.org/uniprot/Q04917
AA Sequence
Gly2-Asn246
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