GCDH Protein, Human, Recombinant (His Tag)
Product Specifications
Overview
A DNA sequence encoding the human GCDH (NP_000150.1) (Met1-Lys438) was expressed with a polyhistidine tag at the C-terminus.
Synonyms
ACAD5 Protein, Human; GCD Protein, Human
Gene Name
Glutaryl-CoA dehydrogenase
Expression System
Baculovirus-Insect Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile 20 mM Tris, pH 8.0, 300 mM NaCl, 10 % glycerol. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant human GCDH consists of 405 amino acids and predicts a molecular mass of 44.9 kDa.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH50717I2.pdf
Symbol
GCDH
Species
Human
Protein ID
NP_000150.1
AA Sequence
Met1-Lys438
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_000150.1
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