FUT8 Protein, Human, Recombinant (aa 68-575, His Tag)

Product Specifications

Background

Alpha (1,6) fucosyltransferase 8, also known as FUT8, is a member of the glycosyltransferase family. Fucosyltransferases are the enzymes transferring fucose from GDP-Fuc to Gal in an alpha1,2-linkage and to GlcNAc in alpha1,3-linkage, alpha1,4-linkage, or alpha1,6-linkage. All fucosyltransferases utilize the same nucleotide sugar, their specificity reside in the recognition of the acceptor and in the type of linkage formed. Fucosyltransferases share some common structural and catalytic features. On the basis of protein sequence similarities, these enzymes can be classified into four distinct families: (1) the alpha-2-fucosyltransferases, (2) the alpha-3-fucosyltransferases, (3) the mammalian alpha-6-fucosyltransferases, and (4) the bacterial alpha-6-fucosyltransferases. The alpha-3-fucosyltransferases constitute a distinct family as they lack the consensus peptide, but some regions display similarities with the alpha-2 and alpha-6-fucosyltranferases.

Overview

A DNA sequence encoding the human FUT8 isoform 1 (Q9BYC5-1) (Arg 68-Lys 575) was fused with a polyhistidine tag at the carboxy-terminus.

Synonyms

MGC26465 Protein, Human

Gene Name

Fucosyltransferase 8 (alpha (1,6) fucosyltransferase)

UniProt

Q9BYC5-1

Expression System

Baculovirus-Insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its ability to hydrolyze the donor substrate GDP fucose.The specific activity is >0.75 pmoles/min/μg.

Form

Liquid

Buffer

Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins

Molecular Weight

The recombinant human FUT8 consists of 518 amino acids and has a calculated molecular mass of 60 kDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

Liquid. It is shipped out with blue ice. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.