TIGIT Protein, Human, Recombinant (His Tag), Biotinylated

Product Specifications

Background

TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM) . TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.

Overview

A DNA sequence encoding the TIGIT (NP_776160.2) (Met1-Phe138) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

Synonyms

VSIG9 Protein, Human; VSTM3 Protein, Human; WUCAM Protein, Human

Gene Name

T cell immunoreceptor with Ig and ITIM domains

Expression System

HEK293 Cells

Tag

C-His

Field of Research

ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

1.Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT His, Biotinylated (Cat:PH50665M2) at 2 μg/ml (100 μl/well) can bind Mouse CD155/PVR His & hFc (Cat:50259-M03H), the ECof Mouse CD155/PVR His & hFc is 50-250 ng/mL. 2. Using the Octet RED System, the affinity constant (Kd) of TIGIT Protein, Human, Recombinant (His Tag), Biotinylated (Cat: PH50665M2) bound to MITG7192A was 0.1 nM.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Biotinylated proteins, Cancer proteins, Receptor Proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins

Molecular Weight

The recombinant TIGIT consists of 128 amino acids and predicts a molecular mass of 14.2 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.