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NOTCH2 Protein, Human, Recombinant (His Tag)

Product Specifications

Background

NOTCH2 (Notch Receptor 2) is a Protein Coding gene. This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics and play a role in a variety of developmental processes by controlling cell fate decisions. Hajdu Cheney Syndrome (HCS) is a rare disease associated with mutations of NOTCH2 that lead to the translation of a truncated, presumably stable, NOTCH2 protein. NOTCH2 is down-regulated in colon cancer, and reduced expression is associated with a less differentiated, more aggressive phenotype, and reduced overall survival. NOTCH2 has also been shown to have pro-apoptotic and growth-suppressive effects in thyroid carcinoma, and carcinoid tumors. NOTCH2 acts as an oncogene that promotes bladder cancer growth and metastasis through EMT, cell-cycle progression, and maintenance of stemness.

Synonyms

AGS2 Protein, Human; HJCYS Protein, Human; hN2 Protein, Human

Gene Name

Notch 2

Expression System

Baculovirus-Insect Cells

Tag

C-His

Field of Research

Notch PathwayTumor Suppressors

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 %glycerol, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Receptor Proteins

Molecular Weight

The recombinant human NOTCH2 consists of 516 amino acids and predicts a molecular mass of 55.5 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Product Datasheet

http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH50658I2.pdf

Symbol

NOTCH2

Species

Human

Protein ID

NP_001186930.1

Overview

A DNA sequence encoding the human NOTCH2 (NP_001186930.1) (Met1-Gln530) was expressed with a polyhistidine tag at the C-terminus.

AA Sequence

Met1-Gln530

Protein ID Link

https://www.ncbi.nlm.nih.gov/protein/NP_001186930.1

Available Sizes

Curated Selection

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