CD32A Protein, Human, Recombinant (167 Arg, His Tag)

Product Specifications

Overview

A DNA sequence encoding (Met 1-Ile 218) of human CD32a (AAA35827.1) was expressed with a C-terminal polyhistidine tag. It is identical to FCGR2A131H/R in the reference.

Synonyms

CD32 Protein, Human; CD32A Protein, Human; CDw32 Protein, Human; Fc gamma RIIA Protein, Human; FCG2 Protein, Human; FcGR Protein, Human; FCGR2 Protein, Human; FCGR2A1 Protein, Human; IGFR2 Protein, Human

Gene Name

Fc fragment of IgG receptor IIa

Expression System

CHO Stable Cells

Tag

C-His

Field of Research

Fc Receptors ITIM/ITAM Immunoreceptors and Related Molecules

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its ability to bind Human IgG1 (Cat:PH50782M0-B), Biotinylated in a functional ELISA.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

CD Molecule proteins, Receptor Proteins, Fc receptor proteins

Molecular Weight

The secreted recombinant human CD32a consists of 196 amino acids and predicts a molecular mass of 22 KDa. The apparent molecular mass of the protein is approximately 29-32 KDa in SDS-PAGE under reducing conditions due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.