Casein Kinase 1 alpha Protein, Human, Recombinant (GST Tag)

Product Specifications

Background

Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to theprotein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at 'Ser-45'. CSNK1A1 may play a role in segregating chromosomes during mitosis.

Overview

A DNA sequence encoding the full length of human CSNK1A1 isoform 1 (P48729-1) (Met 1-Phe 337) was expressed with the GST tag at the N-terminus.

Synonyms

CK1 Protein, Human; CK1a Protein, Human; CKIa Protein, Human; HEL-S-77p Protein, Human; HLCDGP1 Protein, Human; PRO2975 Protein, Human

Gene Name

Casein kinase 1, alpha 1

UniProt

P48729-1

Expression System

Baculovirus-Insect Cells

Tag

N-GST

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by SDS-PAGE.

Activity

The specific activity was determined to be 30 nmol/min/mg using casein as substrate.

Form

Liquid

Buffer

Supplied as sterile 20mM Tris, 500mM NaCl, 2mM GSH, pH 7.4 Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins

Molecular Weight

The recombinant human CSNK1A1/GST chimera consists of 561 amino acids and predicts a molecular mass of 65.2 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.