4-1BB/CD137 Protein, Human, Recombinant (ECD, His Tag)

Product Specifications

Background

CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4and CDT cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials. Soluble forms of CD137 (sCD137) are generated by differential splicing. sCD137 can bind to CD137 ligand to antagonize the costimulatory activities of the membrane-bound CD137 and reduce T cell proliferation and IL-2 secretion.

Overview

A DNA sequence encoding the human 4-1BB extracellular domain (NP_001552.2) (Met 1-Gln 186) was fused with a polyhistidine tag at the C-terminus.

Synonyms

4-1BB Protein, Human; CD137 Protein, Human; CDw137 Protein, Human; ILA Protein, Human

Gene Name

Tumor necrosis factor receptor superfamily, member 9

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Cancer Drug Targets

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human 4-1BB-His (Cat: PH50499M2) at 2 μg/ml (100 μl/well) can bind human 4-1BBL-hFc (Cat: PH52312M3), the ECof human 4-1BBL-hFc is 8-40 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

TNF Superfamily proteins, CD Molecule proteins, Cancer proteins, Receptor Proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins, Cytokine Proteins

Molecular Weight

The recombinant human 4-1BB consists of 174 amino acids and has a calculated molecular mass of 18.8 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of human 4-1BB is approximately 30-35 kDa due to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.