PD-L2 Protein, Human, Recombinant (His Tag), Biotinylated

Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.

A DNA sequence encoding the extracellular domain (Met1-Pro219) of human PD-L2 (NP_079515.2) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

B7-DC Protein, Human; B7DC Protein, Human; bA574F11.2 Protein, Human; Btdc Protein, Human; CD273 Protein, Human; PD-L2 Protein, Human; PDCD1L2 Protein, Human; PDL2 Protein, Human

Measured by its binding ability in a functional ELISA. Immobilized Human PD-1 hFc (Cat:PH51597M4) at 2 μg/ml (100 μl/well) can bind Human PD-L2 His, Biotinylated (Cat:PH50473M2), the ECof Human PD-L2 His, Biotinylated is 300-900 ng/mL.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Biotinylated proteins, B7 Family proteins, CD Molecule proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.