IL25/IL17E Protein, Human, Recombinant (hFc Tag)

Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17–producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP) –activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.

1. Measured by its binding ability in a functional ELISA. Immobilized human IL17BR-His (Cat:PH51330M2) at 10 μg/ml (100 μl/well) can bind human Fc-IL25 (Cat:PH50452M3), The ECof human Fc-IL25 (Cat:PH50452M3) is 1.7-4.2 μg/mL. 2. Measured by its ability to induce CXCL1/GROα secretion in HT29. The EDfor this effect is 15-60 ng/mL.

Lyophilized from sterile PBS , pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The recombinant human IL25/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer consists of 403 amino acids and predicts a molecular mass of 45 KDa. As a result of glycosylation, the apparent molecular mass of rh IL25/Fc monomer is approximately 50-60 KDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the mature form of human IL25 (Tyr 33-Gly177) (NP_073626.1) was fused with the Fc region of human IgG1 at the N-terminus via a polypeptide linker.