CD122/IL2RB Protein, Human, Recombinant (ECD, mFc Tag)

Product Specifications

Background

Interleukin-2 receptor (IL-2R) also known as High-affinity IL-2 receptor subunit beta, IL-2 receptor subunit beta, and IL-2RB, is involved in T cell-mediated immune responses. CD122/IL-2RB is present in 3 forms concerning the ability to bind interleukin 2. The low-affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high-affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high-affinity forms of CD122/IL-2RB are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. CD122/IL-2RB expression was restricted to the earliest B220+ cells (CD43+CD24-; prepro B cells; fraction A) that proliferate vigorously to IL-2 in the absence of any stromal cells, but not to IL-15. The high-affinity form of this receptor is expressed on activated T lymphocytes, activated B lymphocytes, and activated macrophages. CD122/IL-2RB plays a role in regulating normal lymphocyte development.

Overview

A DNA sequence encoding the human IL2RB (NP_000869.1) (Met1-Asp239) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Synonyms

CD122 Protein, Human; IL-15RB Protein, Human; IL15RB Protein, Human; P70-75 Protein, Human

Gene Name

Interleukin 2 receptor, beta

Expression System

HEK293 Cells

Tag

C-mFc

Field of Research

Neuroinflammation

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

1. Measured by its ability to bind biotinylated IL2 basic (Cat:11848-HNAE-B) in a functional ELISA. 2. Measured by its ability to inhibit IL-15-dependent proliferation of MO7e human megakaryocytic leukemic cells in the presence of 4 ng/mL of recombinant human IL-15. The EDfor this effect is typically 2-12 μg/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

CD Molecule proteins, Receptor Proteins, Enzyme Proteins

Molecular Weight

The recombinant human IL2RB consists of 447 amino acids and predicts a molecular mass of 51 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.