HEXA Protein, Human, Recombinant (Subunit A, His Tag)

Product Specifications

Overview

A DNA sequence encoding the human HEXA (AAD13932.1) (Met 1-Thr 529) was fused with a polyhistidine tag at the C-terminus.

Synonyms

TSD Protein, Human

Gene Name

Hexosaminidase A (alpha polypeptide)

Expression System

Baculovirus-Insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MU-Glc-NAc) .The specific activity is >1,250 pmoles/min/μg.

Form

Lyophilized

Buffer

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins

Molecular Weight

The recombinant human HEXA consists of 517 amino acids and predicts a molecular mass of 59.7 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.