CGB5 Protein, Human, Recombinant (mFc Tag)

Product Specifications

Background

The human CGB5 gene encodes chorionic gonadotropin (hCG) beta 5, which is aberrantly expressed in trophoblastic neoplasm and in some non-trophoblastic neoplasms. Fucntional studies observed that it involved tumor initiation, growth, and metastatic outgrowth. Carriage of particular variants in the promoter of the CGB5 gene seems to protect against RM.

Overview

A DNA sequence encoding the human CGB5 (NP_000728.1) (Met1-Gln165) was expressed with the Fc region of mouse IgG1 at the C-terminus.

Synonyms

CGB Protein, Human; CGB5 Protein, Human; HCG Protein, Human; hCGB Protein, Human

Gene Name

Chorionic gonadotropin beta subunit 5

Expression System

HEK293 Cells

Tag

C-mFc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant human CGB5/mFc comprises 379 amino acids and has a predicted molecular mass of 41.9 kDa. The apparent molecular mass of the monomer is approximately 56 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.