VEGF B Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

Vascular endothelial growth factor-B (VEGF-B) is closely related to VEGF-A, an effector of blood vessel growth during development and disease and a strong candidate for angiogenic therapies. In detail, VEGFB can positively prevent the Ang II-induced rising in the size of cardiomyocyte as well as reduce Ang II-induced mRNA and protein levels of β-MHC (β-myosin heavy chain), BNP (brain natriuretic peptide), and ANP (atrial natriuretic peptide) . Moreover, VEGFB can regulate the decline of the Ang II-induced rising in Ca.

Overview

A DNA sequence encoding the human VEGFB (NP_003368.1) (Pro22-Ala207) was expressed with the Fc region of human IgG1 at the N-terminus.

Synonyms

VEGFL Protein, Human; VRF Protein, Human

Gene Name

Vascular endothelial growth factor B

Expression System

HEK293 Cells

Tag

N-hFc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA.Immobilized human S4-Fc3L3-VEGFB at 10 μg/ml (100 μL/well) can bind biotinylated human NRP1-Fc (Cat:PH50251M4), The ECof biotinylated human NRP1-Fc (Cat:PH50251M4) is 0.03-0.07μg/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Growth Factor proteins, Biological Target proteins

Molecular Weight

The recombinant human VEGFB consists 446 amino acids and predicts a molecular mass of 47.8 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.