FGF9 Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

Fibroblast growth factor 9 (FGF9) also known as Glia-activating factor or Heparin-binding growth factor 9, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis. FGF9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. FGF9 may have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

Overview

A DNA sequence encoding the mature form of human fibroblast growth factor 9 (NP_002001.1) (Leu 4-Ser 208) was expressed with the fused Fc region of human IgG1 at the N-terminus.

Synonyms

FGF-9 Protein, Human; GAF Protein, Human; HBFG-9 Protein, Human; HBGF-9 Protein, Human; SYNS3 Protein, Human

Gene Name

Fibroblast growth factor 9

Expression System

HEK293 Cells

Tag

N-hFc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 %, as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The EDfor this effect is typically 10-60 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Growth Factor proteins, Cytokine Proteins, Cell Therapy proteins, Biological Target proteins

Molecular Weight

The recombinant human Fc/FGF9 is a disulfide-linked homodimeric protein. The reduced monomer consists of 463 amino acids and has a predicted molecular mass of 54 and 37 kDa as estimated in SDS-PAGE under reducing conditions duo to glycosylation.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.