MZB1/PERP1 Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

MZB1 (Marginal Zone B And B1 Cell Specific Protein, also known as MEDA-7 and pERp1) is a Protein Coding gene. MZB1 is a B-cell-specific and endoplasmic reticulum (ER) -localized protein implicated in antibody secretion and integrin-mediated cell adhesion. MZB1 is important for B cell function as a key regulator of antibody secretion, calcium homeostasis, and adhesion. MZB1 may play a central role in B cell neoplasms and is a potential target for future therapeutic interventions. Low MZB1 expression was an independent prognostic factor for recurrence after curative gastrectomy and was associated significantly with increased hematogenous recurrence. MZB1 acts as a suppressor of gastric cancer (GC) . Low MZB1 expression in the primary GC tissue is predictive of recurrence after curative resection.

Overview

A DNA sequence encoding the human MZB1 (NP_057543.2) (Met1-Thr185) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

HSPC190 Protein, Human; MEDA-7 Protein, Human; PACAP Protein, Human; pERp1 Protein, Human

Gene Name

Marginal zone B and B1 cell-specific protein

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant human MZB1 consists 404 amino acids and predicts a molecular mass of 44.9 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.