LSAMP Protein, Human, Recombinant (hFc Tag)

The limbic system-associated membrane protein (LAMP) is a cell surface glycoprotein expressed by cortical and subcortical regions of the mammalian CNS that comprise or receive direct projections from limbic system structures. The 64-68-kDa glycoprotein limbic system-associated membrane protein (LsAMP) is expressed on the surface of somata and proximal dendrites of neurons. These areas perform cognitive and autonomic functions, also learning, and memory. The functional analysis indicates that LsAMP acts as a selective adhesion molecule, serving as a guidance cue for specific patterns of connectivity, which underlies the normal development of the limbic system. In animal studies there has been found that rats with an increased level of anxiety had 1.6-fold higher expression of the LsAMP gene in the periaqueductal gray compared to rats with a low level of anxiety, indicating a possible role of LsAMP in the regulation of anxiety.

1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant human OPCML-His (Cat:PH52427M2) at 10 μg/mL can bind recombinant human LSAMP-Fc with a linear range of 31.25-250 ng/ml.

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

The secreted recombinant human LSAMP/Fc is a disulfide-linked homodimer. Yhe reduced monomer consists of 528 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, the apparent molecular mass of rhLSAMP/Fc monomer is approximately 80-85 kDa in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

A DNA sequence encoding the human LSAMP (Q13449) (Met 1-Asn 315), without the pro peptide, was was fused with the Fc region of human IgG1 at the C-terminus.