RNF43 Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

RNF43 mutations are frequently detected in colorectal cancer cells and lead to a loss of function of the ubiquitin E3 ligase. The outer mitochondrial membrane 34 (TOMM34) and ring finger protein 43 (RNF43) as highly expressed oncogenes in malignant colorectal tumors. RNF43 is a tumour suppressor gene that suppresses the Wnt-beta-catenin signalling pathway.

Overview

A DNA sequence encoding the human RNF43 (NP_060233.3) (Met1-Tyr197) was expressed with the Fc region of human IgG1 at the C-terminus.

Synonyms

RNF124 Protein, Human; RNF43 Protein, Human; URCC Protein, Human

Gene Name

Ring finger protein 43

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human RSPO3 His (Cat:PH50710M2) at 2 μg/ml (100 μl/well) can bind Human RNF43 hFc (Cat:PH50273M4), the ECof Human RNF43 hFc is 3.0-12.0 ng/mL

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Molecular Weight

The recombinant human RNF43/Fc comprises 412 amino acids and has a predicted molecular mass of 45.8 kDa. The apparent molecular mass of the protein is approximately 52 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.