What is the catalog number for PGA4 Protein, Human, Recombinant (hFc Tag)?

The catalog number for PGA4 Protein, Human, Recombinant (hFc Tag) is PH50231M4.

What sizes are available for PGA4 Protein, Human, Recombinant (hFc Tag)?

PGA4 Protein, Human, Recombinant (hFc Tag) is available in the following size(s): 50 µg.

Is PGA4 Protein, Human, Recombinant (hFc Tag) in stock?

PGA4 Protein, Human, Recombinant (hFc Tag) is currently made to order / not held in immediate stock. Contact us for lead time.

PGA4 Protein, Human, Recombinant (hFc Tag)

Product Specifications

Background

PGA4 (Pepsinogen 4, group I), or Pepsinogen A, is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed, and does not digest the body's own proteins in the stomach's lining. Five types of zymogens of pepsins, gastric digestive proteinases, are known: pepsinogens A, B, and F, progastricsin, and prochymosin. There are two major groups of pepsinogen, namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin), and each frequently has isozymogens. The PGA3, PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.

Overview

A DNA sequence encoding the human PGA4 (NP_001073276.1) (Met 1-Ala 388) was fused with the Fc region of human IgG1 at the C-terminus.

Synonyms

FLJ58952 Protein, Human; FLJ77962 Protein, Human; PGA3 Protein, Human; PGA4 Protein, Human; PGA5 Protein, Human

Gene Name

Pepsinogen 4, group I (pepsinogen A)

Expression System

HEK293 Cells

Tag

C-hFc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 80 % as determined by SDS-PAGE.

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp) -NH2, AnaSpec, Catalog # 27114.The specific activity is > 3000 pmoles/min/μg. (Activation description: The enzyme achieves its activity under acidic pH)

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins, Protease proteins, Diagnostic proteins

Molecular Weight

The secreted recombinant human PGA4/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 614 amino acids and has a predicted molecular mass of 67.3 kDa. The apparent molecular mass of rhPGA/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.