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FGF1 Protein, Human, Recombinant

Product Specifications

Background

AFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1–4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.

Synonyms

AFGF Protein, Human; ECGF Protein, Human; ECGF-beta Protein, Human; ECGFA Protein, Human; ECGFB Protein, Human; FGF-1 Protein, Human; FGF-alpha Protein, Human; FGFA Protein, Human; GLIO703 Protein, Human; HBGF-1 Protein, Human; HBGF1 Protein, Human

Gene Name

Fibroblast growth factor 1 (acidic)

Expression System

E. coli

Tag

No tag

Endotoxin

Please contact us for more information.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The EDfor this effect is typically 0.15-0.75 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Biological Target proteins, Diagnostic proteins, Growth Factor proteins, Biomarker Proteins, Enzyme Proteins, Cell Therapy proteins

Molecular Weight

The recombinant human FGF acidic consists of 141 amino acids and has a calculated molecular mass of 16 kDa as estimated by SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

Product Datasheet

http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH50214E0.pdf

Symbol

FGF1

Species

Human

Protein ID

AAA79245.1

Overview

A DNA sequence encoding the mature form of human FGF acidic (AAA79245.1) (Phe 16-Asp 155) was expressed, with an additional Met at the N-terminus.

AA Sequence

Phe16-Asp155

Protein ID Link

https://www.ncbi.nlm.nih.gov/protein/AAA79245.1

Available Sizes

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