IL-12 Protein, Human, Recombinant (His Tag)

Product Specifications

Overview

A DNA sequence encoding the human IL12 complex composed of IL12B (NP_002178.2) (Met1-Ser328) and the mature form of human IL12A (P29459-1) (Arg23-Ser219) linked by a polypeptide linker was fused with a polyhistidine tag at the C-terminus.

Synonyms

CLMF Protein, Human; CLMF2 Protein, Human; IL-12A Protein, Human; IL-12B Protein, Human; IL12 Protein, Human; IL12 p70 Protein, Human; IMD28 Protein, Human; IMD29 Protein, Human; NFSK Protein, Human; NKSF Protein, Human; NKSF1 Protein, Human; NKSF2 Protein, Human; P35 Protein, Human

Gene Name

Interleukin 12A

UniProt

P29459-1

Expression System

HEK293 Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The EDfor this effect is 0.2-2 ng/mL.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Biological Target proteins

Molecular Weight

The recombinant human consists 529 amino acids and predicts a molecular mass of 59.6 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.