Frizzled 4 Protein, Human, Recombinant (His Tag)

Product Specifications

Background

Familial exudative vitreoretinopathy (FEVR) is a hereditary blinding disorder that features defects in retinal vascular development. Mutations in FZD4 are known to cause autosomal dominant exudative vitreoretinopathy (EVR1) . The mutations in FZD4 are associated with the phenotypes of retinal folds or ectopic macula in FEVR

Overview

A DNA sequence encoding the human FZD4 (NP_036325.2) (Met1-Glu180) was expressed with a polyhistidine tag at the C-terminus.

Synonyms

CD344 Protein, Human; EVR1 Protein, Human; FEVR Protein, Human; Fz-4 Protein, Human; Fz4 Protein, Human; FZD4S Protein, Human; FzE4 Protein, Human; GPCR Protein, Human; hFz4 Protein, Human

Gene Name

Frizzled class receptor 4

Expression System

HEK293 Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Activity

At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Growth Factor proteins, CD Molecule proteins, Receptor Proteins, Cytokine Proteins, epigenetic proteins

Molecular Weight

The recombinant human FZD4 consists 155 amino acids and predicts a molecular mass of 17.7 kDa.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.