ALK-1 Protein, Human, Recombinant (His Tag)
Product Specifications
Background
Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.
Synonyms
ACVRLK1 Protein, Human; ALK-1 Protein, Human; ALK1 Protein, Human; HHT Protein, Human; HHT2 Protein, Human; ORW2 Protein, Human; SKR3 Protein, Human; TSR-I Protein, Human
Gene Name
Activin A receptor type II-like 1
Expression System
HEK293 Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 92 % as determined by SDS-PAGE.
Activity
Measured by its ability to inhibit BMP9 induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells. David, L. et al. (2007) Blood 109:1953.The EDfor this effect is typically 50-200 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
TGF beta Family proteins, Receptor Proteins, Enzyme Proteins, Cytokine Proteins
Molecular Weight
The recombinant human ALK1 comprises 108 amino acids and has a predicted molecular mass of 12.3 kDa. As a result of glycosylation, rhALK1 migrates as an approximately 27 kDa protein in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH50087M2.pdf
Symbol
ACVRL1
Species
Human
Protein ID
NP_000011.2
Overview
A DNA sequence encoding the the extracellular domain of human ALK1 (NP_000011.2) (Met 1-Gln 118) was fused with a polyhistide tag at the C-terminus.
AA Sequence
Met1-Gln118
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_000011.2
Available Sizes
Curated Selection
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