Granzyme B Protein, Human, Recombinant (His Tag)

Product Specifications

Background

Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 3 substrates of GZMB have been identified including the key substrate caspase-3, ICAD, and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells and accordingly plays an essential role in immunosurveillance.

Overview

A DNA sequence encoding the proform of human Granzyme B (NP_004122.1) (Met 1-Tyr 247) was expressed with a C-terminal polyhistidine tag.

Synonyms

CCPI Protein, Human; CGL-1 Protein, Human; CGL1 Protein, Human; CSP-B Protein, Human; CSPB Protein, Human; CTLA1 Protein, Human; CTSGL1 Protein, Human; HLP Protein, Human; SECT Protein, Human

Gene Name

Granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

Expression System

HEK293 Cells

Tag

C-His

Field of Research

Serine Proteases and Regulators

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 97 % as determined by SDS-PAGE.

Activity

Measured by its ability to cleave a peptide substrate, tButyloxycaronylAlaAlaAspThioBenzylester (BocAADSBzl), in the presence of 5,5’Dithiobis (2nitrobenzoic acid) (DTNB) . The specific activity is >1500 pmol/min/μg. (The enzyme needs to be activated by Recombinant Mouse Active Cathepsin C)

Form

Lyophilized

Buffer

Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Function

Enzyme Proteins, Diagnostic proteins

Molecular Weight

The recombinant human Granzyme B consists of 240 amino acids and has a calculated molecular mass of 27.2 kDa. As a result of glycosylation, the apparent molecular mass of rhGranzyme B is approximately 35-40 kDa in SDS-PAGE under reducing conditions.

Storage Conditions

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.