CHST11 Protein, Human, Recombinant (His Tag)
Product Specifications
Background
CHST11, also known as C4ST-1, belongs to the sulfotransferase 2 family. CHST11 localizes to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. A chromosomal translocation involving CHST11 gene and IgH, t (12;14) (q23; q32), has been reported in a patient with B-cell chronic lymphocytic leukemia.
Synonyms
C4ST Protein, Human; C4ST-1 Protein, Human; C4ST1 Protein, Human; HSA269537 Protein, Human
Gene Name
Carbohydrate (chondroitin 4) sulfotransferase 11
UniProt
Q9NPF2-2
Expression System
Baculovirus-Insect Cells
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Enzyme Proteins
Molecular Weight
The secreted recombinant human CHST11 consists of 323 amino acids and predicts a molecular mass of 38.4 KDa. The apparent molecular mass of the protein is approximately 43 KDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Product Datasheet
http://www.leading-biology.com/en/fastadmin/public/data/datasheet/S_Protein_datasheet/PH50004I2.pdf
Symbol
CHST11
Species
Human
Overview
A DNA sequence encoding the human CHST11 (Q9NPF2-2) (Met36-Glu347) was expressed with a C-terminal polyhistidine tag.
Uniprot URL
https://www.uniprot.org/uniprot/Q9NPF2-2
AA Sequence
Met36-Glu347
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